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There are two main methods that can be used for DNA paternity tests, including PCR (Polymerase Chain Reaction) and RFLP (Restriction Fragment Length Polymorphism). In this article we consider these two methods, the difference is and how they work.

Polymerase Chain Reaction test usually involves taking a swab from inside the cheek for DNA samples. It is faster than RFLP testing and especially look between six and nine loci on the DNA. This test does not give the same level of information provided by the RFLP test.

PCR-DNA test works by "strengthening" of the DNA sample, or take a small DNA sample and then multiplying. This is useful if only a small sample can be obtained and is also useful when working with degraded DNA. When using DNA testing by PCR, however, the laboratory must be particularly careful about preventing pollution in the sample if the amplification process might tend to increase the risk of contamination.

The process of DNA testing by PCR is to heat the DNA, the addition of primers and then recombines it to cool and an enzyme reads the DNA sequence of multiple copies of DNA to create.

The restriction fragment length polymorphism tests is longer than the PCR test and is an old method, but can provide more reliable results, each locus contains more information about paternity. It requires a larger sample of DNA and is more likely to use the blood for DNA testing, but it can also be done using a buffer inside the cheek, if necessary.

The AABB 2004 report said that there was a decrease in the number of laboratories using the RFLP method and an increase in laboratory using PCR DNA testing and PCR is used in 98.34% of cases.

Finally, there are two main methods of DNA paternity tests primarily used today. Both methods are testing gene amplification and restriction fragment length polymorphism testing. PCR is used in 98.34% of cases, DNA tests based on the 2004 AABB and appears to increase over the old method of RFLP. PCR is a faster method of testing and increases the amount of material DNA, so that only small samples are needed, but this leads to a risk of infection. If you opt for a PCR test or order a DNA test kit at home, make sure they are approved by the AABB and they offer a guarantee of at least 99% and test at least ten loci .

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